JOURNAL ARTICLE

Toward the genetic suppression of Bactrocera dorsalis (Diptera: Tephritidae) through CRISPR/Cas9-mediated editing of spermatogenesis-related genes, Tssk1 and topi for imparting male sterility.

  • Published In: Annals of the Entomological Society of America, 2024, v. 117, n. 5. P. 270 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Ashok, Karuppannasamy; Bhargava, Chikmagalur Nagaraja; Pradeep, Chalapathi; Pradhan, Sanjay Kumar; Jha, Girish Kumar; Maligeppagol, Manamohan; Shivanna, Bynakal; Asokan, Ramasamy 3 of 3

Abstract

This article focuses on using CRISPR/Cas9-mediated genome editing to induce male sterility in the oriental fruit fly, *Bactrocera dorsalis*, a significant agricultural pest. The study targeted two spermatogenesis-related genes, Testis-specific zinc finger protein (topi) and Testis-specific serine protein kinase 1 (Tssk1), resulting in males that produced fewer eggs and had lower egg hatching rates when mated with wild females, thereby reducing viable offspring. This approach demonstrates a potential environmentally safer alternative to traditional Sterile Insect Technique (SIT) methods, which rely on irradiation and have associated drawbacks. The findings suggest that CRISPR/Cas9 editing of conserved male fertility genes like topi and Tssk1 could enhance genetic pest control strategies for *B. dorsalis* and possibly other insect pests.

Additional Information

  • Source:Annals of the Entomological Society of America. 2024/09, Vol. 117, Issue 5, p270
  • Document Type:Article
  • Subject Area:Agriculture and Agribusiness
  • Publication Date:2024
  • ISSN:0013-8746
  • DOI:10.1093/aesa/saae021
  • Accession Number:179665348
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