JOURNAL ARTICLE

In vivo biosensing of subcellular pyruvate pools reveals photosynthesis-dependent metabolite dynamics in Nicotiana benthamiana.

  • Published In: Journal of Experimental Botany, 2024, v. 75, n. 22. P. 7254 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Multhoff, Jan; Niemeier, Jan-Ole; Zheng, Ke; Lim, Magdiel Sheng Satha; Barreto, Pedro; Niebisch, Jule Meret; Ischebeck, Till; Schwarzländer, Markus 3 of 3

Abstract

The article focuses on establishing in vivo biosensing of pyruvate, a central metabolite in plant metabolism, using the genetically encoded pyruvate biosensor PyronicSF in Nicotiana benthamiana. The study characterizes the sensor's ratiometric fluorescence properties and pH sensitivity, and demonstrates its functionality in the cytosol, mitochondria, and chloroplasts, revealing dynamic changes in subcellular pyruvate pools during dark-light transitions linked to photosynthetic activity. While stable expression of the sensor in Arabidopsis was hindered by gene silencing, transient expression in N. benthamiana enabled real-time monitoring of pyruvate dynamics with spatial and temporal resolution not achievable by traditional biochemical methods. The work highlights both the potential and current limitations of PyronicSF for dissecting metabolic regulation in living plant tissues.

Additional Information

  • Source:Journal of Experimental Botany. 2024/12, Vol. 75, Issue 22, p7254
  • Document Type:Article
  • Subject Area:Anatomy and Physiology
  • Publication Date:2024
  • ISSN:0022-0957
  • DOI:10.1093/jxb/erae398
  • Accession Number:181289391
  • Copyright Statement:Copyright of Journal of Experimental Botany is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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