Biochemical and functional characterization of skeletal muscle cells differentiated from tonsil‐derived mesenchymal stem cells.

Introduction/Aims: Human tonsils are a readily accessible source of stem cells for the potential treatment of skeletal muscle disorders. We reported previously that tonsil‐derived mesenchymal stem cells (TMSCs) can differentiate into skeletal muscle cells (SKMCs), which renders TMSCs promising candidates for cell therapy for skeletal muscle disorders. However, the functional properties of the myocytes differentiated from mesenchymal stem cells have not been clearly evaluated. In this study we investigated whether myocytes differentiated from TMSCs (skeletal muscle cells derived from tonsil mesenchymal stem cells [TMSC‐SKMCs]) exhibit the functional characteristics of SKMCs. Methods: To test the insulin reactivity of TMSC‐SKMCs, the expression of glucose transporter 4 (GLUT4) and phosphatidylinositol 3‐kinase/Akt was analyzed after the cells were treated for 30 minutes with 100 nmol/L insulin in normal or high‐glucose medium. We also examined whether these cells formed a neuromuscular junction (NMJ) when cocultured with motor neurons, and whether they were stimulated by electrical signals using whole‐cell patch clamping. Results: Skeletal muscle cells derived from tonsil mesenchymal stem cells expressed SKMC markers, such as MYOD, MYH3, MYH8, TNNI1, and TTN, at high levels, and exhibited a multinucleated cell morphology and a myotube‐like shape. The expression of the acetylcholine receptor and GLUT4 was confirmed in TMSC‐SKMCs. In addition, these cells exhibited insulin‐mediated glucose uptake, NMJ formation, and transient changes in cell membrane action potential, all of which are representative functions of human SKMCs. Discussion: Tonsil‐derived mesenchymal stem cells can be functionally differentiated into SKMCs and may have potential for clinical application for the treatment of skeletal muscle disorders. [ABSTRACT FROM AUTHOR]