The nuclear DNA and RNA distribution in Pelomyxa spp. (Amoebozoa, Archamoebae, Pelobiontida) revealed by a simple‐to‐use DAPI/pyronin staining method.

  • Published In: Journal of Eukaryotic Microbiology, 2025, v. 72, n. 1. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Bogolyubov, Dmitry S.; Chistyakova, Ludmila V.; Sokolova, Yuliya Y.; Goodkov, Andrew V. 3 of 3

Abstract

The genus Pelomyxa includes 15 species of anaerobic Archamoebae with remarkable diverse nucleoplasm morphology. Nuclear structures, like chromatin and nucleoli, of several members of the genus was previously identified only based on their ultrastructural similarity to typical structures of somatic cells of higher eukaryotes. Here, we explored an easy‐to‐use, one‐step intravital staining method with DAPI and pyronin to distinguish between DNA and RNA structures in nuclei of unfixed cells of Pelomyxa belevskii and P. stagnalis significantly varying by nuclear organization. Our method can be used for rapid diagnosis of DNA and RNA‐containing structures in species with complex nuclear organization. [ABSTRACT FROM AUTHOR]

Additional Information

  • Source:Journal of Eukaryotic Microbiology. 2025/01, Vol. 72, Issue 1, p1
  • Document Type:Article
  • Subject Area:Anatomy and Physiology
  • Publication Date:2025
  • ISSN:1066-5234
  • DOI:10.1111/jeu.70000
  • Accession Number:183979855
  • Copyright Statement:Copyright of Journal of Eukaryotic Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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