Green and Versatile High-Throughput Microwell Oxidation-Based Spectrophotometric Methods for Determination of Galidesivir in Capsules.

This article focuses on the development and validation of three green microwell spectrophotometric methods (MW-SPMs) for the quantitation of galidesivir (GDV), a broad-spectrum antiviral drug, in its bulk form and capsule dosage. The methods utilize the oxidation of GDV by ammonium metavanadate (AMV), chromium trioxide (CTO), and potassium iodate (PIO) in acidic medium within 96-well plates, enabling high-throughput analysis with absorbance measured at specific wavelengths. The MW-SPMs demonstrated excellent linearity, accuracy, precision, and robustness, with no interference from common pharmaceutical excipients, and were confirmed to be environmentally friendly according to multiple greenness assessment tools. These methods offer advantages including reduced reagent/sample volumes, cost-effectiveness, ease of automation, and use of widely available reagents, making them suitable for routine quality control of GDV formulations.