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Chemical Synthesis of Activity‐Based Ubiquitin Probes for Profiling Arabidopsis thaliana Deubiquitinases.

  • Published In: ChemistrySelect, 2025, v. 10, n. 6. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Guo, Yanyan; Hua, Xiao; Han, Xiaona; Li, Pincheng; Li, Yi‐Ming; Shi, Jing 3 of 3

Abstract

Abnormalities in deubiquitinating enzymes (DUBs) activity can lead to growth defects and reduced ability to respond to environmental stress in Arabidopsis thaliana (A. thaliana). However, there is currently a lack of adequate tools to profile active DUBs in plants such as A. thaliana. Here, we developed Ub ABPs that could be efficiently prepared to label A. thaliana DUBs. A total of 7 DUBs in plant lysates were labelled and identified in combination with label‐free quantification (LFQ) mass spectrometry. This work demonstrated the universality of ABP tools in plants and mammals. [ABSTRACT FROM AUTHOR]

Additional Information

  • Source:ChemistrySelect. 2025/02, Vol. 10, Issue 6, p1
  • Document Type:Article
  • Subject Area:Biology
  • Publication Date:2025
  • ISSN:2365-6549
  • DOI:10.1002/slct.202404927
  • Accession Number:183600537
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