JOURNAL ARTICLE

Detection and quantification of SARS-CoV-2 in wastewater using a mobile laboratory setting.

  • Published In: Letters in Applied Microbiology, 2025, v. 78, n. 1. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Mathur, Shubham; Salah, Ikram; Msarwa, Haneen; Craddock, Hillary A; Moran-Gilad, Jacob 3 of 3

Abstract

This article evaluates the GeneXpert point-of-care testing (POCT) device for rapid detection of SARS-CoV-2 in wastewater as part of wastewater-based epidemiology (WBE). Conducted in Israel from June to September 2022, the study compared GeneXpert's performance to an established in-house RT-qPCR method across laboratory, field, and mobile testing environments using 151 wastewater samples. Results showed 100% agreement in the laboratory, 90% agreement in field testing, and 62.5% detection in mobile testing, with GeneXpert providing results in under an hour and a detection limit of approximately 12 copies per milliliter. While promising for rapid, deployable surveillance—especially in remote or resource-limited settings—the study notes technical challenges in mobile contexts and calls for further validation across diverse wastewater systems and operational conditions.

Additional Information

  • Source:Letters in Applied Microbiology. 2025/01, Vol. 78, Issue 1, p1
  • Document Type:Article
  • Subject Area:Biology
  • Publication Date:2025
  • ISSN:0266-8254
  • DOI:10.1093/lambio/ovae135
  • Accession Number:182849322
  • Copyright Statement:Copyright of Letters in Applied Microbiology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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