DIGESTION OF DNA WITH RESTRICTION ENZYMES (ECOR1 AND HIND III) OF HIBISCUS SABDARIFFA.

The term medicinal plants include a various type of plants used in herbalism and some of these plants have a medicinal activity. These medicinal plants consider as a rich source of ingredients which can be used in drug development and synthesis. Hibiscus sabdariffa belonging to family Malvaceae is used very important herbaceous crop which can be consumed for improvement in health condition due to its nutritional value. It contains minerals like potassium, phosphorus, sodium, iron, etc, which are medicinally and commercially important. Medicinal uses of these plant includes the property of antioxidant, anti-cancerous, antimicrobial activity etc. It can be used for curing Anaemia as well as diuretic problems. It is commonly known as Roselle. In the present work, restriction digestion pattern has been studied in the DNA isolated from the leaves of Hibiscus sabdariffa which will help in analysing the docking sites for molecular work. Excellent quality of DNA was recovered and used for Restriction digestion. Complete digestion provided with EcoRI and HindIII resulted in 6 and 4 fragment which conforms the presence of 5 restriction site of EcoRI and 3 Site for HindIII.anti-human immunodeficiency virus (HIV), antimalarial, antioxidant, antiviral, antifungal, anticonvulsant, and antibacterial are only some of the pharmacological effects. [ABSTRACT FROM AUTHOR]