JOURNAL ARTICLE

Clonal expression and structural analysis of polylactic acid-degrading enzyme S8SP from Bacillus safensis.

  • Published In: Letters in Applied Microbiology, 2025, v. 78, n. 3. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Wang, Yujun; Zhang, Yuanyi; Li, Chunwang; Meng, Siqi; Wang, Dandan; Zhao, Lehui; Wang, Zhanyong 3 of 3

Abstract

This article focuses on the identification and characterization of S8 serine peptidase (S8SP), a polylactic acid (PLA)-degrading enzyme from Bacillus safensis. The enzyme was heterologously expressed in Escherichia coli and demonstrated the ability to degrade PLA films, causing surface erosion and increased hydrophilicity under optimal conditions of 40°C and pH 8.0. S8SP exhibits stability within moderate temperature and pH ranges, is activated by sodium and potassium ions, and contains a classical hydrolase catalytic triad (Asp138, His170, Ser327) conserved among Bacillus species. These findings suggest that S8SP has potential applications in PLA waste recycling and contribute to the understanding of PLA-degrading enzymes in environmental biodegradation.

Additional Information

  • Source:Letters in Applied Microbiology. 2025/03, Vol. 78, Issue 3, p1
  • Document Type:Article
  • Subject Area:Chemistry
  • Publication Date:2025
  • ISSN:0266-8254
  • DOI:10.1093/lambio/ovaf025
  • Accession Number:184348656
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