JOURNAL ARTICLE

A Simple UV Spectrophotometric Method Based on Continuous Wavelet Transform and Multivariate Calibration Model for the Concurrent Analysis of Three Water-Soluble Vitamins in Fertility Supplements.

  • Published In: Journal of AOAC International, 2024, v. 107, n. 1. P. 164 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Lohrdy, Mahla; Sohrabi, Mahmoud Reza; Davallo, Mehran 3 of 3

Abstract

This article focuses on the development and validation of a rapid, cost-effective, and eco-friendly spectrophotometric method combined with chemometric techniques—continuous wavelet transform (CWT) and partial least squares (PLS)—for the simultaneous determination of water-soluble vitamins B6 (pyridoxine), B12 (cobalamin), and C (ascorbic acid) in fertility supplements for men and women. The study demonstrates that both CWT and PLS methods accurately quantify these vitamins without requiring time-consuming extraction or organic solvents, showing high recovery rates (above 95%), low limits of detection, and strong agreement with high-performance liquid chromatography (HPLC) results, as confirmed by analysis of variance (ANOVA) tests. The proposed approaches offer advantages in simplicity, speed, reduced solvent use, and lower cost, suggesting their suitability as alternatives to traditional chromatographic methods in quality control laboratories.

Additional Information

  • Source:Journal of AOAC International. 2024/01, Vol. 107, Issue 1, p164
  • Document Type:Article
  • Subject Area:Complementary and Alternative Medicine
  • Publication Date:2024
  • ISSN:1060-3271
  • DOI:10.1093/jaoacint/qsad093
  • Accession Number:174766053
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