JOURNAL ARTICLE

Preparation of low lactose prebiotic milk using β‐galactosidase from lotus seeds (Nelumbo nucifera).

  • Published In: International Journal of Food Science & Technology, 2023, v. 58, n. 5. P. 2609 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Zhong, Ailing; Katrolia, Priti; Zhu, Chong; Kopparapu, Narasimha Kumar 3 of 3

Abstract

This article focuses on the isolation, purification, and characterization of a novel β‐galactosidase enzyme, named NelnuGal, from lotus seeds (Nelumbo nucifera). NelnuGal is a 50 kDa monomeric enzyme optimally active at pH 4.0 and 50 °C, exhibiting broad pH stability (4–10), thermostability (20–50 °C), and resistance to inhibition by metal ions such as Ca²⁺ and Mg²⁺. Notably, NelnuGal is not inhibited by high concentrations of galactose, a common end-product inhibitor of β‐galactosidases, and demonstrates both lactose hydrolysis and transgalactosylation activities, producing galactooligosaccharides (GOS). The study further shows that adding lotus seed powder to milk and whey effectively reduces lactose content while enriching GOS, suggesting potential applications in the dairy industry for lactose-intolerant consumers and prebiotic food development.

Additional Information

  • Source:International Journal of Food Science & Technology. 2023/05, Vol. 58, Issue 5, p2609
  • Document Type:Article
  • Subject Area:Complementary and Alternative Medicine
  • Publication Date:2023
  • ISSN:0950-5423
  • DOI:10.1111/ijfs.16413
  • Accession Number:163049688
  • Copyright Statement:Copyright of International Journal of Food Science & Technology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Looking to go deeper into this topic? Look for more articles on EBSCOhost.