The In-vitro Antioxidant and Anti-inflammatory Action of Aloe Vera, Chamomile, and Propolis: A Formulation Analysis.

Aims & Background: The aim of this research is to evaluate the Antioxidant and Anti-inflammatory activity of Aloe vera, Chamomile, and Propolis formulation. Plant extracts have been widely used for their therapeutic properties for many centuries. Polyphenols are the primary compounds present in the Aloe vera, Chamomile, and Propolis which provides anti-oxidant and anti-inflammatory activity and are responsible for offering immunity against free radical tissue damage and are crucial in preventing various chronic diseases such as inflammation, anaemia, cardiovascular disorders and aging. Materials and Methods: The aqueous Aloe vera, Chamomile, and Propolis formulation was prepared. The antioxidant capacity of the formulation was determined using 2 different assays such as DPPH and H2O2 assays. The anti-inflammatory capability of the formulation was assessed using two assays: BSA and EA assays. Results: The inhibitory percentage of DPPH scavenging activity varied from 65%-90% at a concentration range between 10-50 μg/mL. The inhibitory percentage of H2O2 scavenging activity varied from 49%-85% at a concentration range between 10-50 μg/mL. The inhibitory percentage of heat-induced BSA denaturation ranged from 45%-80%. The protein denaturation inhibition ranged from 50%-80% at a concentration range between 10-50 μg/mL. The percentage of inhibition of egg albumin denaturation assay ranged from 55%-80%. Conclusion: The results obtained from the assays proved that Aloe vera, Chamomile, and Propolis formulations possess potential antioxidant and anti-inflammatory capacity and can be effectively used as an effective antioxidant agent and anti-inflammatory agent. In order to increase the efficacy, it is important to combine components that have increased therapeutic properties. Clinical significance: These findings support the present usage of Aloe vera, Chamomile, and Propolis formulation as an effective alternative for treating various diseases. [ABSTRACT FROM AUTHOR]