Network Pharmacology Based Study on the Mechanism of Mongolian Medicine Valeriana officinalis L. on Liver Cancer.

[Objectives] To explore the mechanism of Mongolian medicine Valeriana officinalis L. on liver cancer by network pharmacology. [Methods] The HERB database of V. officinalis L. was searched, and the Uniprot database was used to normalize and standardize the targets. Liver cancer targets were searched through GeneCards, DISGENET, and other databases. Venny website was used to obtain the intersection target of valerian active ingredients and liver cancer disease. The protein-protein interaction (PPÏ) network of the intersection targets was analyzed by STRING database, and the PPI network was constructed by Cytoscape software. The David database was used for GO functional annotation and KEGG pathway enrichment analysis to obtain the relevant pathways in the treatment of liver cancer with Mongolian medicine V. officinalis. The corresponding chemical components, targets and pathways of liver cancer were imported into Cytoscape software to construct the network topology of "chemical component-disease-target-pathway". According to the analysis results, the potential of the active components in V. officinalis as a therapeutic drug for liver cancer was evaluated, and the correlation between the results of network pharmacology analysis and clinical treatment of liver cancer was discussed, which provided a reference for clinical application. [Results] A total of 13 kinds of chemical components and 108 drug disease intersection target genes were screened, and the core genes acting on diseases were caffeic acid, perillyl acetate, (+)-alpha-Terpineol, eucalyptol, etc.; GO functional enrichment analysis involved 389 items of biological processes, 62 items of cellular components and 120 items of molecular functions. Enrichment analysis of KEGG signaling pathways screened out chemical carcinogenesis-receptor activation, cancer pathways, prolactin signaling pathways, proteoglycans in cancer, EGFR tyrosine kinase inhibitor resistance and other signaling pathways. [Conclusions] The mechanism of Mongolian medicine V. officinalis on liver cancer was studied by network pharmacology. It was found that it can inhibit the proliferation of liver cancer cells from multiple targets and pathways. This is expected to provide a theoretical basis for further basic experimental research. [ABSTRACT FROM AUTHOR]