JOURNAL ARTICLE

Large‐scale separation of alkaloids from Corydalis decumbens by pH‐zone‐refining centrifugal partition chromatography and their anticomplement activity.

  • Published In: Journal of Separation Science, 2024, v. 47, n. 1. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Xie, Xin‐Xin; Chen, Zhong‐Jian; Zhu, Quan‐Gang; Yu, Qin; Lian, Tian‐Yan; Xu, Xue‐Lian; Chen, Ya; Song, Wei‐Hua 3 of 3

Abstract

Centrifugal partition chromatography in the pH‐zone‐refining mode was successfully applied to the separation of alkaloids from the crude extract of Corydalis decumbens. The experiment was performed with a two‐phase solvent system composed of petroleum ether‐ethyl acetate‐ethanol‐water (5:5:3:7, v/v/v/v) where triethylamine (10 mM) was added to the stationary phase and hydrochloric acid (10 mM) to the mobile phase. From 1.6 g of the crude extract, 43 mg protopine, 189 mg (+)‐egenine, and 158 mg tetrahydropalmatine were obtained with a purity of 98.2%, 94.6%, and 96.7%, respectively. Tetrahydropalmatine showed an interesting anticomplement effect with CH50 0.11 and AP50 0.25 mg/mL, respectively. In a mechanistic study, tetrahydropalmatine interacted with C1, C3, C4, and C5 components in the complement activation cascade. [ABSTRACT FROM AUTHOR]

Additional Information

  • Source:Journal of Separation Science. 2024/01, Vol. 47, Issue 1, p1
  • Document Type:Article
  • Subject Area:Complementary and Alternative Medicine
  • Publication Date:2024
  • ISSN:1615-9306
  • DOI:10.1002/jssc.202300577
  • Accession Number:174881392
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