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Protective effect of probiotic (Lactobacillus casei) against Escherichia coli causing diarrhea.

  • Published In: Misan Journal of Academic Studies, 2025, v. 24, n. 56. P. 56 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Turki, Ali J.; Kareem, Dhuhaa Kh.; Alsheikly, Abeer M. 3 of 3

Abstract

Based on the significant impact of L. casei against E. coli obtained from diarrheal case in several Baghdadi hospitals (Yarmouk and Mahmoudia), this study was conducted. Every sample that was grown on MacConkey agar, Eosine Methylene Blue, and Blood agar, as well as E. coli, was identify using Gram stain and biochemical testing. Forty-five isolates of the fifty diarrheal samples had positive E. coli results. Sensitivity tests were performed on the four E. Coli isolates that were obtained and came back positive Lactobacillus casei isolates were isolated and cultur in Man Rogosa Sharpe broth at 37°C for 24 hours, followed by a 24-hour incubation under CO2 (5-10%) and a reculture on MRS agar. The isolates were tested against E. coli using the agar well diffusion assay, and the diameter of the halo zone surrounding bacterial growths was measured . [ABSTRACT FROM AUTHOR]

Additional Information

  • Source:Misan Journal of Academic Studies. 2025/12, Vol. 24, Issue 56, p56
  • Document Type:Article
  • Subject Area:Complementary and Alternative Medicine
  • Publication Date:2025
  • ISSN:1994-697X
  • DOI:10.54633/2333-024-056-006
  • Accession Number:191875836
  • Copyright Statement:Copyright of Misan Journal of Academic Studies is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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