JOURNAL ARTICLE

Nested PCR Targeting the Flagellin Gene (fliC) in Urine Sample for Diagnosis of Typhoid Fever - An Internal Pilot Study.

  • Published In: QJM: An International Journal of Medicine, 2024, v. 117. P. ii207 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Fouad, Mona Mohammad; Fathy, Maha Muhammad; Mohammed Fathi, Marwa Saad; El-Folly, Runia Fouad; Khater, Walaa Shawky 3 of 3

Abstract

The article focuses on evaluating the use of nested polymerase chain reaction (PCR) targeting the flagellin gene (fliC) of Salmonella enterica serotype Typhi in urine samples for diagnosing typhoid fever. Conducted as an internal pilot study with 25 clinically diagnosed patients and 25 healthy controls in Cairo, Egypt, the nested PCR showed a sensitivity of 44% and specificity of 88%, with a positive predictive value of 78.57%. Blood culture demonstrated a low positivity rate of 4%. The study concludes that while nested PCR of urine samples has moderate sensitivity, its higher specificity indicates potential as a supplementary diagnostic tool for typhoid fever, warranting further validation before routine clinical use.

Additional Information

  • Source:QJM: An International Journal of Medicine. 2024/10, Vol. 117, pii207
  • Document Type:Article
  • Subject Area:Consumer Health
  • Publication Date:2024
  • ISSN:1460-2725
  • DOI:10.1093/qjmed/hcae175.482
  • Accession Number:181636101
  • Copyright Statement:Copyright of QJM: An International Journal of Medicine is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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