Characterization and Biological Activity of Silver Nanoparticles from (Rhizophora Mucronata) Mangrove Extract.

In this study, silver nanoparticles (AgNPs) were biosynthesized from a crude leaf extract of Rhizophora mucronata. The initial phytochemical analysis of the sample revealed the presence of tannins, steroids, terpenoids, saponins and flavonoids which contribute to the reduction and stabilization of silver ions during the synthesis process. Characterization techniques, including FTIR, UV–Vis spectroscopy, SEM and EDS, confirmed the successful synthesis of AgNPs with distinctive properties. The UV–Vis spectra exhibited an absorption peak around 420 nm, characteristic of AgNPs. SEM images revealed the crystal nature of the nanoparticles, indicating structural stability. The agar well-diffusion method was employed to determine the zone of inhibition against different pathogens. The leaf extract demonstrated the highest antibacterial potential, with substantial zones of inhibition observed against all tested pathogens. The potent anti-inflammatory activity showed the minimum and maximum inhibition of protein denaturation at concentration of 25 μ g/mL and 100 μ g/mL are 28% and 69%, respectively. The silver nanoparticles demonstrated anti-inflammatory properties by inhibiting protein denaturation, suggesting their potential therapeutic applications. The mangrove R. mucronata leaf extract decided to be a favorable reducing agent for the biological synthesis of silver nanoparticles with potent antimicrobial activity. [ABSTRACT FROM AUTHOR]