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A Critical View on the Use of DNA Hydrogels in Cell‐Free Protein Synthesis.

  • Published In: Angewandte Chemie, 2025, v. 137, n. 2. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Moench, Svenja; Lemke, Phillip; Hansen, Abbey; Bickmann, Christoph; Peng, Martin; Rabe, Kersten S.; Domínguez, Carmen M.; Niemeyer, Christof M. 3 of 3

Abstract

Numerous studies have reported in the past that the use of protein‐encoding DNA hydrogels as templates for cell‐free protein synthesis (CFPS) leads to better yields than the use of conventional templates such as plasmids or PCR fragments. Systematic investigation of different types of bulk materials from pure DNA hydrogels and DNA hydrogel composites using a commercially available CFPS kit showed no evidence of improved expression efficiency. However, protein‐coding DNA hydrogels were advantageously used in microfluidic reactors as immobilized templates for repetitive protein production, suggesting that DNA‐based materials offer potential for future developments in high‐throughput profiling or rapid in situ characterization of proteins. [ABSTRACT FROM AUTHOR]

Additional Information

  • Source:Angewandte Chemie. 2025/01, Vol. 137, Issue 2, p1
  • Document Type:Article
  • Subject Area:Health and Medicine
  • Publication Date:2025
  • ISSN:0044-8249
  • DOI:10.1002/ange.202414480
  • Accession Number:184019976
  • Copyright Statement:Copyright of Angewandte Chemie is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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