P- glycoprotein (P-gp) Mediated Drug Interaction between Digoxin & Orange Juice - An Exploratory Study by in-silico Approach.

Background - The aim of this study explores the potential interaction between digoxin and orange juice through a P-glycoprotein (P-gp)-mediated pathway using in-silico methods. The primary goals are to identify components in orange juice that inhibit P-gp, potentially reducing digoxin's serum toxicity, and to compare the effectiveness of these components with standard Pgp inhibitors such as ketoconazole. Methods - Molecular docking studies were performed to assess the binding affinity of various orange juice constituents to P-gp. The binding energies of these components, including hesperidin, were evaluated, and the highest affinity was determined. Vicenin-26”-O-glucoside from orange juice demonstrated the highest binding affinity to P-gp, suggesting its strong inhibitory potential. Results - The results indicate that orange juice may serve as a moderate but effective P-gp inhibitor, comparable to ketoconazole. Conclusion - Orange juice, particularly through its constituent vicenin-26”-O-glucoside, shows potential as a P-gp inhibitor and could reduce digoxin toxicity, offering a natural alternative to existing pharmaceutical inhibitors like ketoconazole. [ABSTRACT FROM AUTHOR]