JOURNAL ARTICLE

Ruminal Protozoa Viability Under Diverse Additives Using Trypan Blue Exclusion Method as an Alternative Counting Methodology.

  • Published In: Journal of Animal Science, 2023, v. 101. P. 269 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Cuervo, Wilmer; Gomez Lopez, Camila; Fernandez Marenchino, Ignacio; Maderal, Araceli B.; Vargas, Juan; Tarnonsky, Federico; Fernandez-Lehman, Andres; Schulmeister, Tessa M.; Podversich, Federico; DiLorenzo, Nicolas 3 of 3

Abstract

The article focuses on evaluating the Trypan Blue Exclusion Method (TBEM) as an alternative technique to assess ruminal protozoa counts (PROT) and viability (VIAB) in beef cattle, which are linked to methane (CH4) emissions. Two experiments tested TBEM under different culture conditions and feed additives, including yeast fermentation products, tannins, cashew nutshell extract, and citral. Results showed that TBEM effectively detected changes in protozoa viability and counts, correlating reductions in protozoa with decreased methane production and gas output. The study concludes that TBEM is a simple, rapid, and cost-effective method to evaluate protozoa viability and membrane integrity, offering advantages over traditional methyl-green-formalin-saline fixation methods.

Additional Information

  • Source:Journal of Animal Science. 2023/11, Vol. 101, p269
  • Document Type:Article
  • Subject Area:Health and Medicine
  • Publication Date:2023
  • ISSN:0021-8812
  • DOI:10.1093/jas/skad281.322
  • Accession Number:173680779
  • Copyright Statement:Copyright of Journal of Animal Science is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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