Clinical Importance of limnoid Kulactone from Azadirachta indica.

Majority of global population up to 80 percent, relies on traditional medicine for their primary health care. In current phyto-therapy based medical practices, native medicinal plant/herbal extracts are employed to treat various disorders and diseases. Their application is rapidly increasing due to their positive effects and furthermore recognized with low toxic side effects. Azadirachta indica (Neem) is a key medicinal plant with rich medicinal values. It is in cosmopolitan in distribution and belongs to the family of Meliaceae. In India its usage is very common in the native ethno traditional medicinal practices along with other medical practices too. Its vegetative parts like leaves, flowers, fruits, seeds and bark are goldmine for bio-active compounds. Even sometimes fondly called as natural drug store or store house of phytochemicals. Moreover, neem chemical and structural composition is tricky and complex. A small fraction of bioactive compounds are well characterized, and demonstrated with potential biological properties. Moreover previous studies revealed the therapeutic potential of Limoniod kulactone, separated from Melia Species. However, in the present study, we assessed the distribution pattern of Limoniods such as kulactone in the neem extracts of various vegetative parts by LC-MS (Liquid Chromatography and Mass Spectroscopy) spectral analysis. The end results predicted the presence of Limoniod kulactone C30H44O3 (Mass 452.67 m/z) for the first time from the vegetative parts of Azadirachta indica which was collected from Tirumala Hills, Eastern Ghats, India which has clinical significance. [ABSTRACT FROM AUTHOR]