JOURNAL ARTICLE

Quantitative effects of bilirubin photoisomers on the measurement of direct bilirubin by the enzymatic bilirubin oxidase method.

  • Published In: Annals of Clinical Biochemistry, 2026, v. 63, n. 2. P. 152 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Kawaguchi, Nana; Koyano, Kosuke; Morita, Hirosuke; Pengiran Mohamad Fadly, Dk Nur Rosyiidah Apryll Czarina; Shinabe, Yuta; Noguchi, Yuta; Arioka, Makoto; Nakao, Yasuhiro; Ozaki, Miyo; Nakamura, Shinji; Kondo, Sonoko; Konishi, Yukihiko; Kuboi, Toru; Okada, Hitoshi; Yasuda, Saneyuki; Itoh, Susumu; Murao, Koji; Kusaka, Takashi 3 of 3

Abstract

This article focuses on the impact of bilirubin photoisomers on direct bilirubin (DB) measurements using the bilirubin oxidase method, particularly in neonates who have elevated unconjugated bilirubin levels. The study quantitatively analyzed how bilirubin configurational isomers (BCIs) and bilirubin structural isomers (BSIs), generated by light exposure, interfere with DB values by using irradiated neonatal serum samples and high-performance liquid chromatography (HPLC). Results showed that approximately 11% of BCIs and 32% of BSIs were measured as DB, leading to significant overestimation of conjugated bilirubin concentrations. These findings highlight the clinical importance of considering photoisomer interference and careful sample handling when interpreting DB measurements in neonatal care to avoid misdiagnosis of hyperconjugated bilirubinemia.

Additional Information

  • Source:Annals of Clinical Biochemistry. 2026/03, Vol. 63, Issue 2, p152
  • Document Type:Article
  • Subject Area:Health and Medicine
  • Publication Date:2026
  • ISSN:0004-5632
  • DOI:10.1177/00045632251367245
  • Accession Number:192308325
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