JOURNAL ARTICLE

Rapid Characterization and Identification of the Chemical Constituents in San-Huang-Xie-Xin-Tang, a Traditional Chinese Medicine Formula, by High Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

  • Published In: Journal of Chromatographic Science, 2025, v. 63, n. 4. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Liu, Lei; Hu, Pei; Chen, Mengmo; Huang, Jiasi; Zhang, Qing; Ling, Yun 3 of 3

Abstract

This article focuses on the comprehensive chemical profiling of San-Huang-Xie-Xin-Tang (SHXXT), a traditional Chinese medicine formula composed of Coptidis rhizome (CR), Scutellariae radix (SR), and Rhei rhizome (RR). Using high-performance liquid chromatography coupled with electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS), 45 compounds were identified, including 8 phenolic acids, 15 flavonoids, 13 alkaloids, 8 anthraquinones, and 1 lignan, with specific compounds traced to each herbal component. The study demonstrates that HPLC-ESI-QTOF-MS/MS is an effective and sensitive method for rapid and systematic analysis of complex constituents in SHXXT, providing a chemical foundation for further pharmacological research and quality control of this traditional formula.

Additional Information

  • Source:Journal of Chromatographic Science. 2025/04, Vol. 63, Issue 4, p1
  • Document Type:Article
  • Subject Area:Health and Medicine
  • Publication Date:2025
  • ISSN:0021-9665
  • DOI:10.1093/chromsci/bmae056
  • Accession Number:185428169
  • Copyright Statement:Copyright of Journal of Chromatographic Science is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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