JOURNAL ARTICLE

RP-HPLC Method Development and Validation for Simultaneous Estimation of Berberine and Ursolic Acid in Formulation.

  • Published In: Journal of Chromatographic Science, 2025, v. 63, n. 4. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Khanvilkar, Vineeta V; Mandle, Shruti S; Hande, Prachi R; Daka, Bhavin R; Jadhav, Pratik P 3 of 3

Abstract

The article focuses on the development and validation of a Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method for the simultaneous quantitative estimation of berberine and ursolic acid, two active markers in the Ayurvedic formulation Giloy Tulsi tablets. Using a Hemochrom C18 column and a methanol:acetonitrile (4:1, v/v) mobile phase at 292 nm detection wavelength, the method achieved specific, accurate, precise, and robust separation of both markers with retention times of approximately 1.5 and 3.2 minutes, respectively. Validation according to International Conference on Harmonization (ICH) Q2(R1) guidelines confirmed the method's linearity, sensitivity, precision, accuracy, and robustness, supporting its suitability for routine quality control of Ayurvedic or herbal formulations containing these phytoconstituents.

Additional Information

  • Source:Journal of Chromatographic Science. 2025/04, Vol. 63, Issue 4, p1
  • Document Type:Article
  • Subject Area:Health and Medicine
  • Publication Date:2025
  • ISSN:0021-9665
  • DOI:10.1093/chromsci/bmaf016
  • Accession Number:185428174
  • Copyright Statement:Copyright of Journal of Chromatographic Science is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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