JOURNAL ARTICLE

A plasmid containing the human metallothionein-II gene selectively distinguishes trivalent lanthanum from several divalent heavy metal cations during monoclonal antibody-assisted agarose gel electrophoresis.

  • Published In: Toxicology & Industrial Health, 2024, v. 40, n. 1/2. P. 69 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Wooten, Dennis C 3 of 3

Abstract

This article focuses on developing a method to detect and assess the bioavailability of trivalent lanthanide ions using electrophoretic mobility shifts of a plasmid containing the human metallothionein-II (hMT-II) gene. The study demonstrates that the pUC57-MT2 plasmid, when combined with a monoclonal antibody targeting the metal-responsive transcription factor-1 (MTF-1) protein, exhibits distinct and lanthanide-specific mobility patterns in 0.8% agarose gels. These patterns allow differentiation of lanthanum ions from divalent metal ions such as zinc, cobalt, and nickel, suggesting potential applications in industrial, environmental, and biomedical contexts for identifying or separating lanthanide ions without requiring complex equipment or cell cultures.

Additional Information

  • Source:Toxicology & Industrial Health. 2024/01, Vol. 40, Issue 1/2, p69
  • Document Type:Article
  • Subject Area:Health and Medicine
  • Publication Date:2024
  • ISSN:0748-2337
  • DOI:10.1177/07482337231222354
  • Accession Number:175081832
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