JOURNAL ARTICLE

Production of polyclonal antibodies against leek yellow stripe virus (LYSV) coat protein expressed in Escherichia coli and its application in serological diagnostics.

  • Published In: Letters in Applied Microbiology, 2023, v. 76, n. 2. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Gupta, Nitika; Rai, Richa; Islam, Sabina; Meena, R P; Baranwal, V K 3 of 3

Abstract

This article focuses on the development and application of specific polyclonal antisera against Leek yellow stripe virus (LYSV), a significant potyvirus affecting garlic crops worldwide, including India. Using the recombinant coat protein (CP) of LYSV expressed in a bacterial system, researchers produced polyclonal antibodies in rabbits, which were validated through western blotting, dot immunobinding assay (DIBA), immunosorbent electron microscopy (ISEM), and antigen coated plate enzyme-linked immunosorbent assay (ACP-ELISA). The developed antisera demonstrated high specificity and sensitivity, detecting LYSV in 76% of tested garlic accessions without cross-reactivity to related potyviruses like onion yellow dwarf virus (OYDV). This study represents the first report of polyclonal antiserum production against in vitro expressed LYSV-CP and highlights its potential for routine virus indexing in garlic germplasm, tissue culture plantlets, and quarantine programs in India.

Additional Information

  • Source:Letters in Applied Microbiology. 2023/02, Vol. 76, Issue 2, p1
  • Document Type:Article
  • Subject Area:Health and Medicine
  • Publication Date:2023
  • ISSN:0266-8254
  • DOI:10.1093/lambio/ovac064
  • Accession Number:162824652
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