Lipid production by robust Aspergillus oryzae BCC7051 and a mathematical model describing its growth and lipid phenotypic traits.

This article focuses on identifying and characterizing a robust oleaginous strain of the filamentous fungus Aspergillus oryzae, specifically strain BCC7051, for enhanced lipid and biomass production. Comparative analysis of 13 A. oryzae strains revealed that BCC7051 exhibited superior lipid-rich biomass accumulation across a range of pH (3.5–6.5) and temperature (24–42°C) conditions, with glucose identified as the optimal carbon source for lipid production. A mathematical kinetic model incorporating growth and lipid production parameters was developed and validated to predict fungal performance under varying temperatures and carbon substrates. The study concludes that A. oryzae BCC7051 is a promising, economically feasible microbial cell factory for producing lipid-based products and serves as a robust chassis for further strain optimization using modern synthetic biology approaches.