JOURNAL ARTICLE

Design, fabrication, and characterization of picowell arrays on cyclic olefin copolymer surfaces generated with a 10.5 MeV N4+ ion microbeam.

  • Published In: Applied Physics Letters, 2023, v. 123, n. 5. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Bányász, I.; Rajta, I.; Havránek, V.; Mackova, A.; Laki, A. J.; Kellermayer, M. S. Z.; Szittner, Z.; Kurunczi, S.; Novák, Sz.; Székács, I.; Horváth, R.; Fried, M.; Nagy, G. U. L. 3 of 3

Abstract

This article focuses on a novel method for fabricating picoliter-scale well arrays by direct writing on ZEONOR™ cyclo-olefin copolymer surfaces using a high-energy medium-mass ion microbeam. The technique produced a 9 × 9 array of chalice-shaped picowells with an average volume of approximately 820 femtoliters, suitable for hosting single living cells, and demonstrated that the ion implantation did not alter the material's microhardness. The applicability of these picowells in biomedical single-cell assays was validated by successfully positioning fluorescently labeled HeLa cancer cells within the wells. The method offers advantages such as rapid, one-step fabrication with precise control over well geometry and depth, and potential scalability through replication techniques, positioning it as a competitive alternative to laser-based direct writing for microdevice production.

Additional Information

  • Source:Applied Physics Letters. 2023/07, Vol. 123, Issue 5, p1
  • Document Type:Article
  • Subject Area:Life Sciences
  • Publication Date:2023
  • ISSN:0003-6951
  • DOI:10.1063/5.0155681
  • Accession Number:169786978
  • Copyright Statement:Copyright of Applied Physics Letters is the property of American Institute of Physics and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Looking to go deeper into this topic? Look for more articles on EBSCOhost.