JOURNAL ARTICLE

Rapid Quantitative Analysis of 19 Bioactive Components in Fangji Huangqi Decoction Based on UHPLC–MS/MS.

  • Published In: Journal of Chromatographic Science, 2023, v. 61, n. 9. P. 852 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Cui, Wenbo; Yang, Liu; Zhang, Lichao; Liu, Yuetao; Yan, Yan; Li, Aiping; Qin, Xuemei 3 of 3

Abstract

This article focuses on the development and validation of a rapid ultra-high-performance liquid chromatography coupled with Qtrap mass spectrometry (UHPLC-Qtrap-MS) method for simultaneous quantitative analysis of 19 major chemical constituents in Fangji Huangqi Decoction (FHD), a traditional Chinese medicine prescription recorded in "Jin Gui Yao Lue." The method accurately identifies and quantifies compounds including flavonoids, saponins, alkaloids, and lactones from the six herbal components of FHD, with demonstrated high sensitivity, precision, repeatability, and recovery. Multivariate statistical analyses revealed that the chemical profiles of FHD vary according to the source and cultivation patterns of Astragalus membranaceus, a key herb in the formula, supporting distinctions between different batches. This validated analytical approach provides a comprehensive basis for quality control, pharmacological research, and pharmacokinetic studies of FHD.

Additional Information

  • Source:Journal of Chromatographic Science. 2023/10, Vol. 61, Issue 9, p852
  • Document Type:Article
  • Subject Area:Nutrition and Dietetics
  • Publication Date:2023
  • ISSN:0021-9665
  • DOI:10.1093/chromsci/bmac085
  • Accession Number:173495093
  • Copyright Statement:Copyright of Journal of Chromatographic Science is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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