JOURNAL ARTICLE

CRISPR-Cas12a-based genome editing and transcriptional repression for biotin synthesis in Pseudomonas mutabilis.

  • Published In: Journal of Applied Microbiology, 2023, v. 134, n. 3. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Zhao, Jiarun; Zuo, Siqi; Huang, Lei; Lian, Jiazhang; Xu, Zhinan 3 of 3

Abstract

This article focuses on the development of a dual-function CRISPR-Cas12a system for efficient genome editing and transcriptional repression in Pseudomonas mutabilis ATCC 31014. The system, composed of two plasmids expressing Cas12a and CRISPR RNA (crRNA), achieved over 90% efficiency in single-gene deletion, replacement, and inactivation within five days, and enabled simultaneous gene knockout and repression using full-length and truncated crRNAs, respectively. Application of this system to metabolic engineering increased biotin production by 3.84-fold through deletion of the yigM gene and repression of birA. This dual-functional CRISPR-Cas12a tool offers a powerful approach for multiplex metabolic engineering and construction of microbial cell factories in P. mutabilis.

Additional Information

  • Source:Journal of Applied Microbiology. 2023/03, Vol. 134, Issue 3, p1
  • Document Type:Article
  • Subject Area:Nutrition and Dietetics
  • Publication Date:2023
  • ISSN:1364-5072
  • DOI:10.1093/jambio/lxad049
  • Accession Number:162824033
  • Copyright Statement:Copyright of Journal of Applied Microbiology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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