JOURNAL ARTICLE

Characterization of a new Saccharomyces cerevisiae isolated from banana stems and its mutant with l-leucine accumulation for awamori brewing.

  • Published In: Bioscience, Biotechnology & Biochemistry, 2023, v. 87, n. 2. P. 240 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Masatoshi Tsukahara; Shota Isogai; Haruna Azuma; Keiko Tsukahara; Yoichi Toyokawa; Hiroshi Takagi 3 of 3

Abstract

This article focuses on the isolation and characterization of a novel Saccharomyces cerevisiae yeast strain, 35a14, from banana stems in Okinawa, Japan, and its mutant with enhanced l-leucine accumulation for improving awamori brewing. Strain 35a14 is genetically distinct from industrial yeast strains and produces high ethanol and 4-vinyl guaiacol, a flavor precursor in awamori, but lower isoamyl acetate, a key fruity aroma compound. Through mutagenesis, a mutant strain BNNL80 was developed that accumulates higher intracellular l-leucine due to a mutation (Asp578Asn) in the LEU4 gene encoding α-isopropylmalate synthase, leading to increased production of isoamyl acetate and isoamyl alcohol in awamori fermentation. These findings suggest that combining natural yeast isolation with targeted breeding can enhance flavor profiles in traditional distilled beverages like awamori.

Additional Information

  • Source:Bioscience, Biotechnology & Biochemistry. 2023/02, Vol. 87, Issue 2, p240
  • Document Type:Article
  • Subject Area:Nutrition and Dietetics
  • Publication Date:2023
  • ISSN:0916-8451
  • DOI:10.1093/bbb/zbac185
  • Accession Number:161613184
  • Copyright Statement:Copyright of Bioscience, Biotechnology & Biochemistry is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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