JOURNAL ARTICLE

A pilot study to directly estimate radiation-induced mutation in large Japanese field mouse duo sample, mother and offspring, excluding unknown father, using ddRAD sequencing.

  • Published In: Radiation Protection Dosimetry, 2024, v. 200, n. 16-18. P. 1647 1 of 3

  • Database: Applied Science & Technology Source Ultimate 2 of 3

  • Authored By: Ishiniwa, Hiroko; Endoh, Daiji; Tamaoki, Masanori; Onuma, Manabu 3 of 3

Abstract

This article focuses on assessing DNA mutation rates in wild large Japanese field mice (Apodemus speciosus) living in radiation-contaminated areas near the Fukushima Daiichi Nuclear Power Plant following the 2011 nuclear accident. Using double-digest restriction site-associated DNA sequencing (ddRAD-seq) and an optimized enzyme pair (KpnI/PstI), the study compared mutation incidences between pregnant females and their fetuses collected from contaminated and control sites. Results showed no significant increase in mutation rates in fetuses compared to mothers, suggesting limited germline mutation accumulation despite radiation exposure. The study demonstrates the utility of ddRAD-seq for mutation analysis in non-model organisms but notes limitations in selectively targeting functionally important coding regions.

Additional Information

  • Source:Radiation Protection Dosimetry. 2024/11, Vol. 200, Issue 16-18, p1647
  • Document Type:Article
  • Subject Area:Physics
  • Publication Date:2024
  • ISSN:01448420
  • DOI:10.1093/rpd/ncae190
  • Accession Number:180905407
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