Simultaneous EYFP-CENH3/H2B-DsRed Expression Is Impaired Differentially in Meristematic and Differentiated Nuclei of Arabidopsis Double Transformants.

  • Published In: Cytogenetic & Genome Research, 2023, v. 163, n. 1/2. P. 74 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Schubert, Veit; Weißleder, Andrea; Lermontova, Inna 3 of 3

Abstract

Fluorescence live-cell microscopy is important in cell biology to perform artifact-free investigations. To analyze the dynamics of chromatin and centromeres at different stages of the cell cycle in nuclei and chromosomes, we performed simultaneous EYFP-CENH3/H2B-DsRed and single H2B-YFP transformations in Arabidopsis wild-type and cohesin T-DNA mutants. All constructs were under the control of the strong CaMV 35S promoter. While a strong silencing of fluorescence expression occurred differently in leaf and root tissues in the double transformants, nearly all single-transformed wild-type and most mutant cells showed H2B-YFP fluorescence. It seems that for an efficient co-expression of two fluorescence proteins, endogenous promoters and terminators should be used. [ABSTRACT FROM AUTHOR]

Additional Information

  • Source:Cytogenetic & Genome Research. 2023/01, Vol. 163, Issue 1/2, p74
  • Document Type:Article
  • Subject Area:Science
  • Publication Date:2023
  • ISSN:1424-8581
  • DOI:10.1159/000533317
  • Accession Number:173807109
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