Exploring methandienone metabolites generated via homogenized camel liver: Advancements for anti‐doping applications through High Resolution‐Liquid Chromatography Mass Spectrometry analysis.

  • Published In: Rapid Communications in Mass Spectrometry: RCM, 2024, v. 38, n. 22. P. 1 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Vazhat, Ramees Abdulla; Farook, N. A. Mohamed; Nalakath, Jahfar; Komathu, Praseen Ondern 3 of 3

Abstract

Rationale: Anabolic steroids, also known as anabolic–androgenic steroids (AAS), encompass steroidal androgens such as testosterone, as well as synthetic counterparts with similar structures and effects. The misuse of AAS has increased over the years, leading to ethical and welfare concerns in sports. The World Anti‐Doping Agency (WADA) and the International Federation for Equestrian Sports (FEI) have banned AAS in relevant sports. Methandienone is one of the most identified anabolic androgenic steroids in sports drug testing, Therefore, reliable detection methods are crucial for effective doping control and maintaining the integrity of the sports. Methods: This study explores the use of homogenized camel liver for detecting methandienone metabolites in camels. The biotransformation pathways of methandienone in homogenized camel liver tissues are analyzed using Liquid Chromatography‐High Resolution Mass Spectrometry (LC‐HRMS) to identify and characterize the phase I and phase II metabolites. Chromatographic separation was achieved using a Thermo‐Hypersil C18 column. Results: The study has identified 11 methandienone metabolites (M1–M11), this includes 10 phase I and one phase II metabolite. A glucuronic acid conjugate of methandienone was observed in this study, but no sulfonic acid conjugations were found. The metabolites and their possible chemical structures, along with their fragmentation patterns are confirmed using MSMS (MS2) experiments in data‐independent acquisition (DIA) mode. Conclusions: These findings serve as a vital tool for the rapid detection of methandienone, combating its illicit use in camel racing. Comprehensive screenings covering both the parent drug and its metabolites are recommended to improve detection accuracy and ensure regulatory compliance in sports doping. Future research should explore methandienone's metabolite profile in administered camel samples. [ABSTRACT FROM AUTHOR]

Additional Information

  • Source:Rapid Communications in Mass Spectrometry: RCM. 2024/11, Vol. 38, Issue 22, p1
  • Document Type:Article
  • Subject Area:Sports and Leisure
  • Publication Date:2024
  • ISSN:0951-4198
  • DOI:10.1002/rcm.9902
  • Accession Number:180109932
  • Copyright Statement:Copyright of Rapid Communications in Mass Spectrometry: RCM is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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