JOURNAL ARTICLE

Degradation of DDT by γ-hexachlorocyclohexane dehydrochlorinase LinA.

  • Published In: Bioscience, Biotechnology & Biochemistry, 2024, v. 88, n. 1. P. 123 1 of 3

  • Database: Academic Search Ultimate 2 of 3

  • Authored By: Habibullah, Kafayat Olaide Yusuf; Ren Ito; Leonardo Stari; Kouhei Kishida; Yoshiyuki Ohtsubo; Eiji Masai; Masao Fukuda; Keisuke Miyauchi; Yuji Nagata 3 of 3

Abstract

This article focuses on the discovery that γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase LinA, an enzyme from the γ-HCH-degrading bacterium Sphingobium japonicum UT26, can convert the pesticide DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) into its metabolite DDE (1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene). Although LinA’s DDT degradation activity is weak and undetectable in UT26 cells expressing LinA constitutively, a linA-deletion mutant of UT26 engineered to overexpress LinA showed measurable DDT degradation, indicating that elevated LinA expression enables DDT biodegradation. The study suggests that LinA, which requires no cofactors and functions under aerobic conditions, holds potential for constructing effective DDT biodegradation systems, especially when combined with pathways for further degradation of DDE.

Additional Information

  • Source:Bioscience, Biotechnology & Biochemistry. 2024/01, Vol. 88, Issue 1, p123
  • Document Type:Article
  • Subject Area:Zoology
  • Publication Date:2024
  • ISSN:0916-8451
  • DOI:10.1093/bbb/zbad141
  • Accession Number:174455754
  • Copyright Statement:Copyright of Bioscience, Biotechnology & Biochemistry is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Looking to go deeper into this topic? Look for more articles on EBSCOhost.